LOCUS       40924_5299       12201 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway vector pB4nRGW DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa 
            T, Mano S.
  TITLE     Gateway Vectors for Simultaneous Detection of Multiple 
            Protein-Protein Interactions in Plant Cells Using Bimolecular 
            Fluorescence Complementation
  JOURNAL   PLoS ONE 11 (8), E0160717 (2016)
  PUBMED    27490375
REFERENCE   2  (bases 1 to 12201)
  AUTHORS   Mano S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for 
            Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 12201)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12201)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2013) Contact:Shoji Mano National Institute for Basic 
            Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji, 
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     These colnes were obtained at our laboratory.
FEATURES             Location/Qualifiers
     source          1..12201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     341..357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        872..1217
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1238..1732
                     /label=nmrfp1
                     /note="nmrfp1"
     CDS             1241..1270
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1742..1866
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1919..1949
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2003..2659
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHSCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3004..3306
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3350..3474)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3500..3752
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3786..3802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3810..3826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3834..3864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3879..3900)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3934..4186)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4579..5367)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5391..5574)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5780..5804)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6332..7120
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7367..7955
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8141..8281)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8625..8819)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8888..9952)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10389..11015)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      361 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      421 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      481 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      541 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      601 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      661 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      721 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      781 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      841 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      901 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      961 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1021 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1081 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1141 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1201 ttcatttgga gagaacacgg gggactctag aatgagcatg gaacaaaagc taatctccga
     1261 ggaagacttg atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg
     1321 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1381 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     1441 ctgggacatc ctgtcccctc agttcacgta cggctccaag gcctacgtga agcaccccgc
     1501 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1561 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1621 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1681 gaagaagacc atgggctggg aggcctccac cgagcggatg taccccgagg acgctgttat
     1741 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     1801 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     1861 actatggcgg cccctcgagg cctcgagccc tcgaggggcc gcattaggca ccccaggctt
     1921 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1981 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     2041 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     2101 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     2161 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     2221 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcact cttgttacac
     2281 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     2341 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     2401 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     2461 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2521 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2581 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2641 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     2701 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2761 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2821 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2881 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2941 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt
     3001 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     3061 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     3121 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     3181 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     3241 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     3301 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     3361 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     3421 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgataac
     3481 ctagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     3541 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     3601 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     3661 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     3721 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa
     3781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3901 ggctgaatta ggcgcgccta tttctgaatt cccgatctag taacatagat gacaccgcgc
     3961 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     4021 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
     4081 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
     4141 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg atcatccggg
     4201 tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg tgcatctcgg
     4261 tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgggcccg atcatattgt
     4321 cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt cgtaatgata tcggcacctt
     4381 cgaccgcctg ttccgcagag atcccgtggg cgaagaactc cagcatgaga tccccgcgct
     4441 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     4501 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     4561 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     4621 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     4681 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     4741 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     4801 atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga gcctggcgaa
     4861 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     4921 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     4981 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     5041 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     5101 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     5161 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     5221 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     5281 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     5341 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccagatccgg tgcagattat
     5401 ttggattgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     5461 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     5521 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     5581 gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     5641 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatga attcagaaat
     5701 aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     5761 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     5821 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     5881 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     5941 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     6001 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     6061 tggacataag cctcgttcgg ttcgtaagct gtaatgcaag tagcgtaact gccgtcacgc
     6121 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     6181 gcttcttgtt atgacatgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     6241 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     6301 gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc
     6361 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     6421 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     6481 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     6541 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     6601 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     6661 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     6721 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     6781 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     6841 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     6901 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     6961 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     7021 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     7081 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc
     7141 gttcaagccg acgccgcttc gccggcgtta actcaagcga ttagatgcac taagcacata
     7201 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7261 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7321 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7381 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7441 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7501 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7561 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7621 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7681 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7741 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7801 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7861 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7921 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7981 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8041 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8101 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8161 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8221 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8281 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8341 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8401 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8461 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8521 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     8581 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8641 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8701 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     8761 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     8821 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     8881 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     8941 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9001 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9061 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9121 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9181 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9241 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9301 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9361 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9421 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9481 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9541 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9601 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9661 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9721 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     9781 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     9841 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     9901 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc
     9961 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
    10021 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
    10081 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
    10141 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
    10201 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
    10261 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
    10321 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
    10381 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
    10441 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
    10501 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
    10561 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
    10621 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
    10681 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
    10741 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
    10801 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
    10861 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
    10921 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
    10981 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
    11041 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
    11101 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
    11161 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
    11221 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
    11281 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
    11341 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
    11401 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
    11461 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
    11521 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
    11581 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
    11641 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
    11701 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
    11761 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
    11821 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
    11881 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
    11941 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
    12001 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
    12061 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
    12121 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
    12181 gaggggttgg atcaaagtac t
//
